Closed Wendy361 closed 1 year ago
These sequences are not suitable for MSA by muscle or any other algorithm, they are far too long and are surely not globally alignable. Please open an issue with the authors of CRISPRCasFinder to figure out if this is really a muscle issue, and if so capture the input sequences file and muscle command line so that I can reproduce the problem.
Hi thank you for your anwswer. The error was gone when I used a fasta file containing shorter sequences from Illumina. The previous one is the Nanopore long sequence fasta.
Hi I got the following error message when I use the CRISPRCasFinder, which includes MUSCLE as an integrated software. How can I fix this issue?![image](https://user-images.githubusercontent.com/54411033/234616690-144e0938-ce0c-4e40-8981-29fd8b22a5e6.png)