search

rcedgar / muscle

Multiple sequence and structure alignment with top benchmark scores scalable to thousands of sequences. Generates replicate alignments, enabling assessment of downstream analyses such as trees and predicted structures.
https://drive5.com/muscle
GNU General Public License v3.0
186 stars 21 forks source link

*** Error in `muscle': double free or corruption (out): 0x00007ffdb8176090 *** #57

Closed Wendy361 closed 1 year ago

Wendy361 commented 1 year ago

Hi I got the following error message when I use the CRISPRCasFinder, which includes MUSCLE as an integrated software. How can I fix this issue? image

rcedgar commented 1 year ago

These sequences are not suitable for MSA by muscle or any other algorithm, they are far too long and are surely not globally alignable. Please open an issue with the authors of CRISPRCasFinder to figure out if this is really a muscle issue, and if so capture the input sequences file and muscle command line so that I can reproduce the problem.

Wendy361 commented 1 year ago

Hi thank you for your anwswer. The error was gone when I used a fasta file containing shorter sequences from Illumina. The previous one is the Nanopore long sequence fasta.