Multiple sequence and structure alignment with top benchmark scores scalable to thousands of sequences. Generates replicate alignments, enabling assessment of downstream analyses such as trees and predicted structures.
also, I think it is unlikely sequences of length ~300k are globally alignable, I can't think of an example of biological sequences this long which don't have re-arrangement events
Command used:
muscle -align 1.fasta -output alignment.fasta
Error information: muscle 5.1.linux64 [] 528Gb RAM, 56 cores Built Feb 24 2022 03:16:15 (C) Copyright 2004-2021 Robert C. Edgar. https://drive5.com
WARNING: 7 duplicate labels
Input: 241 seqs, avg length 324490, max 2766791
00:00 133Mb CPU has 56 cores, running 24 threads Segmentation fault (core dumped)riors