rcedgar
commented
4 months ago As suggested by the warning message, these sequences are much too long, they are surely not suitable for global multiple alignment. R
On Tue, Apr 16, 2024 at 1:26 PM m-leitman @.***> wrote:
Hi! This is my first time using muscle. I am on a macOS with the arm64 version. I ran the command ./muscle -super5 all_complete_cut.fa -output alfn.afa. The output is as follows: muscle 5.1.osxarm64 [ef62d8-dirty] 17.2Gb RAM, 8 cores Built Jan 15 2022 11:59:50 (C) Copyright 2004-2021 Robert C. Edgar. https://drive5.com
Input: 5301 seqs, length avg 52390 max 372876
WARNING: Sequence length >5k may require excessive memory
00:02 337Mb 100.0% Derep 5263 uniques, 37 dupes 00:02 342Mb CPU has 8 cores, running 8 threads
./muscle -super5 all_complete_cut.fa -output alfn.afa Elapsed time 00:02 Max memory 342Mb
---Fatal error--- allocflat.cpp(15) assert failed: uint64(Size) == Size64
What is going wrong?
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Hi! This is my first time using muscle. I am on a macOS with the arm64 version. I ran the command ./muscle -super5 all_complete_cut.fa -output alfn.afa. The output is as follows: muscle 5.1.osxarm64 [ef62d8-dirty] 17.2Gb RAM, 8 cores Built Jan 15 2022 11:59:50 (C) Copyright 2004-2021 Robert C. Edgar. https://drive5.com
Input: 5301 seqs, length avg 52390 max 372876
WARNING: Sequence length >5k may require excessive memory
00:02 337Mb 100.0% Derep 5263 uniques, 37 dupes 00:02 342Mb CPU has 8 cores, running 8 threads
./muscle -super5 all_complete_cut.fa -output alfn.afa Elapsed time 00:02 Max memory 342Mb
---Fatal error--- allocflat.cpp(15) assert failed: uint64(Size) == Size64
What is going wrong?