I noticed the following in your instructions for training RDP.
"Based on our experience, trimming the sequences to a specific region does not improve accuracy."
I wanted to inform you that I explicitly tested this some years ago, and in my experience trimming did have a significant impact on assignment accuracy.
Metabarcoding free-living marine nematodes using curated 18S and CO1 reference sequence databases for species-level taxonomic assignments, DOI: 10.1002/ece3.4814
I have only ever used trimmed training sets since then and would advise other users to do the same.
Dear Team,
I noticed the following in your instructions for training RDP.
"Based on our experience, trimming the sequences to a specific region does not improve accuracy."
I wanted to inform you that I explicitly tested this some years ago, and in my experience trimming did have a significant impact on assignment accuracy.
Metabarcoding free-living marine nematodes using curated 18S and CO1 reference sequence databases for species-level taxonomic assignments, DOI: 10.1002/ece3.4814
I have only ever used trimmed training sets since then and would advise other users to do the same.
Kind regards,
Lara