Image restoration: QRBF is brightfield deconvolution of PSD, Mexico

[JohnTigue]

"B.G.-M. and M.d.J.S.M. are inventors on a patent application that describes QRBF."

• 2014 Direct Imaging of Phase Objects Enables Conventional Deconvolution in Bright Field Light Microscopy
• 2017 Quantitative Image Restoration in Bright Field Optical Microscopy. Basically convolution with a kernel like subfig A?

[JohnTigue]

Just terminology here:

From the fluorescence crowd:

Z stacks are usually required for deconvolution of epi-fluorescence microscopy data to remove the out-of-focus signal collected within each individual image.

So, we're working with brightfield, not fluorescence but out-of-focus is out of focus.

[JohnTigue]

Get the code going where the anti-PSF kernel is a parameter. It could be a theoretical function or it could have been determined experimentally.

[JohnTigue]

Maybe two tools here: one uses point spread function to filter for boutons and synapses (more point like) and the second tool uses a line spread function to filter for axons and dendrites.

Test polystyrene objects should be calibrated in size to match desired resolver (ball size of synapse, wire size or axon).

[JohnTigue]

Sounds a lot like Microscopy: Deconvolution Microscopy (David Agard).

[JohnTigue]

JFT on twitter:

And when it comes to addressing PSFs in a brightfield microscopy setting, this seems to be the cutting edge (this is 2014; they have a 2017(?) later paper further developing this work.

With link to Direct Imaging of Phase Objects Enables Conventional Deconvolution in Bright Field Light Microscopy

[JohnTigue]

"super-resolution microscopy, called single-point edge-excitation sub-diffraction (SPEED)"

SPEED Microscopy: Fast Single-molecular Tracking and 3D Deconvolution Process

[JohnTigue]

https://indiagoneviral.com/science/2020/05/05/imaging-technology-allows-visualization-of-nanoscale-structures-inside-whole-cells-2/51382/india-gone-viral/