roozbehdn
commented
8 months ago Hi Thomas, Sorry for our late response. Yes, you can run SPLASH on R1 fastq files and it should work for detecting differential splicing between two groups.
Open tomsing1 opened 10 months ago
roozbehdn
commented
8 months ago Hi Thomas, Sorry for our late response. Yes, you can run SPLASH on R1 fastq files and it should work for detecting differential splicing between two groups.
Context: I have paired-end RNA-seq data from a stranded library preparation protocol. I would like to identify differential splicing events between two groups (including cryptic exons). Is it possible / useful to use both the R1 and R2 reads in the analysis? Many thanks for making SPLASH available - and congratulations on the Cell paper!