Vary the point spread function across channels

reillytilbury / coppafish

Python version of iss software

MIT License

4 stars 2 forks source link

Vary the point spread function across channels #263

Closed paulshuker closed 3 months ago

paulshuker commented 3 months ago

A spot in different image channels may show up as a different radius. If this is the case, the PSF should vary between channels to correct for this. This should make the spot shapes more uniform across all channels, allowing for hopefully better OMP scoring, for example. This will need to be investigated more.

paulshuker commented 3 months ago

Looking at various average spot shapes post-filtering across different channels, there is minimal difference in shape. So, this feature will not be added.