I ran a short test on skewer and I got an empty sequence in the output file.
I know this case is not supposed to happen since I tried to remove an adapter supposed to be located at the read head, but in fact it is at the tail.
But maybe it would be nice remove the header too?
Here is the input file I used to test the adapters filtering:
Hello everyone,
I ran a short test on skewer and I got an empty sequence in the output file. I know this case is not supposed to happen since I tried to remove an adapter supposed to be located at the read head, but in fact it is at the tail. But maybe it would be nice remove the header too?
Here is the input file I used to test the adapters filtering:
I launched skewer with the following command line: skewer -x TTTTTTTTTTTTTTTTTTTT -1 -l 0 -k 15 -t 16 -m head input.fasta >output.fasta
Here is the output fasta file I obtained:
Thanks for your help!