Hi,
I have been using skewer for demultiplexing with the command /home/projects/cu_10049/apps/skewer/skewer -b -z -t 10 -x primers_forward.fa -y primers_reverse.fa file_R1.fastq.gz fil_R2.fastqz -o file --quiet and I get the following output in the log file:
206344 read pairs processed; of these:
0 ( 0.00%) short read pairs filtered out after trimming by size control
0 ( 0.00%) empty read pairs filtered out after trimming by size control
206344 (100.00%) read pairs available; of these:
132384 (64.16%) assigned read pairs available after processing
73960 (35.84%) unassigned read pairs available after processing
Barcode dispatch after trimming:
category count percentage:
A01 0 0.00%
A02 0 0.00%
A03 0 0.00%
A04 0 0.00%
A05 0 0.00%
A06 0 0.00%
A07 0 0.00%
A08 0 0.00%
A09 0 0.00%
A10 0 0.00%
A11 0 0.00%
A12 0 0.00%
A13 0 0.00%
A14 1 0.00%
A15 1 0.00%
A16 1 0.00%
B01 311 0.23%
B02 403 0.30%
However, when I look at the output there are sequences in the files for A02 and multiple of the other AXX's (but none in A01). On the other hand, I get no sequences for B01, but I do get 403 for B02. Can you help me resolve this issue?
Hi, I have been using skewer for demultiplexing with the command
/home/projects/cu_10049/apps/skewer/skewer -b -z -t 10 -x primers_forward.fa -y primers_reverse.fa file_R1.fastq.gz fil_R2.fastqz -o file --quietand I get the following output in the log file:However, when I look at the output there are sequences in the files for A02 and multiple of the other AXX's (but none in A01). On the other hand, I get no sequences for B01, but I do get 403 for B02. Can you help me resolve this issue?