replikation / poreCov

SARS-CoV-2 workflow for nanopore sequence data
https://case-group.github.io/
GNU General Public License v3.0
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Guppy sequ kit #105

Closed DataSpott closed 3 years ago

DataSpott commented 3 years ago

Thought about possibility of adding other barcode-kits, but looking through them no others looked really relevant. Only one I missed in nanozoo/guppy-container was the guppy .cfg-file for the 96-rapid barcoding kit. Couldn´t find information about this file so far either.

replikation commented 3 years ago
DataSpott commented 3 years ago

Implemented support for Rapid-barcoding-kit. Update to guppy-gpu (and therefore also guppy-cpu) -container rolled back, due to issues between guppy v.4.5.4 and CUDA with following error-message in .command.log:

ONT Guppy basecalling software version 4.5.4+66c1a7753
config file:        /opt/ont/guppy/data/dna_r9.4.1_450bps_hac.cfg
model file:         /opt/ont/guppy/data/template_r9.4.1_450bps_hac.jsn
input path:         20210423_covid_rapBar_test2
save path:          fastq_tmp
chunk size:         2000
chunks per runner:  512
minimum qscore:     9
records per file:   4000
num basecallers:    4
gpu device:         auto
kernel path:        
runners per device: 4

[guppy/error] main: CUDA error at /builds/ofan/ont_core_cpp/ont_core/common/cuda_common.cpp:203: CUDA_ERROR_COMPAT_NOT_SUPPORTED_ON_DEVICE
[guppy/warning] main: An error has occurred. Aborting.