Closed omarkr8 closed 2 years ago
arent they using the ARTIC V3 primers?
the primers that are included are listed here: https://github.com/replikation/poreCov/tree/master/data/external_primer_schemes/nCoV-2019
you can also check directly if these match to what you have
oh it seems the midnight kit uses the V1200 primers.
I notice the 0.11.0 version added V4 primers, and I also see that the 1200bp primers have been around before this.
Im thinking the V4 primers are the ones used in the nanopore midnight kit EXP-MRT001 ? does porecov account for the rapid barcoding step (variable read lengths)? I guess just adjusting length filters according would work.
sorry if this is written elsewhere and ive just missed it.