Closed JaMaack closed 2 years ago
since the error is from:
Running: artic-tools check_vcf --summaryOut barcode22.vcfreport.txt barcode22.merged.vcf.gz external_primer_schemes/nCoV-2019/VarSkipV2/nCoV-2019.scheme.bed 2> barcode22.vcfcheck.log
Command failed:artic-tools check_vcf --summaryOut barcode22.vcfreport.txt barcode22.merged.vcf.gz external_primer_schemes/nCoV-2019/VarSkipV2/nCoV-2019.scheme.bed 2> barcode22.vcfcheck.log
can you visually inspect if all the files from the artic-tools check_vcf
are present in /home/minion/Sequenzierung/Analysis/poreCov/results/20220328-01/alignment_data/work/15/5de05280883000c5dd16c97d531a15
my first guess would be that maybe something is wrong with this file (e.g. not enough reads or something).
usually, we ignore these errors by default. i just noticed that this "ignore" was deactivated and I forgot to activate this so the workflow is not failing if some less good barcodes are fed into artic
The /home/minion/Sequenzierung/Analysis/poreCov/results/20220328-01/alignment_data/work/15/5de05280883000c5dd16c97d531a15
folder contains these files/folders (via ls command):
20220328-01 barcode22_filtered.fastq.gz.index barcode22.merged.vcf.gz barcode22.nCoV-2019_2.vcf barcode22.primertrimmed.rg.sorted.bam.bai barcode22.trimmed.rg.sorted.bam.bai
barcode22.alignreport.er barcode22_filtered.fastq.gz.index.fai barcode22.merged.vcf.gz.tbi barcode22.primersitereport.txt barcode22.sorted.bam barcode22.vcfcheck.log
barcode22.alignreport.txt barcode22_filtered.fastq.gz.index.gzi barcode22.minion.log.txt barcode22.primers.vcf barcode22.sorted.bam.bai external_primer_schemes
barcode22_filtered.fastq.gz barcode22_filtered.fastq.gz.index.readdb barcode22.nCoV-2019_1.vcf barcode22.primertrimmed.rg.sorted.bam barcode22.trimmed.rg.sorted.bam sequencing_summary_FAT41443_4eceb848.txt
`
Looks like barcode22.vcfreport.txt
isn't present.
okay probably not enough reads or coverage. the next release will ignore these errors again so it wont fail (is currently in the testing phase). for now I recommend excluding the barcode. We had a few exit 20 errors previously in older poreCov versions and it was always due to bad samples (reads too short, not enough coverage, too many human reads and not enough viral, etc.)
Is there some way to exclude a specific barcode via the command line?
@JaMaack not really. the new porecov release will be out today in a few hours. this would automatically ignore and skip this barcode. if you want it now you can update porecov and run the latest master:
# update
nextflow pull replikation/poreCov
# run poreCov via master
nextflow run replikation/poreCov -r master <your commands>
Thanks, I'll update poreCov and repeat the runs later.
1.4.1
available now
Hi, we recently had problems with some of our runs. The issue seems to be with the nanopolishing for barcode22 samples. After this, the runs stops with an error message and we have no idea why.
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