replikation / poreCov

SARS-CoV-2 workflow for nanopore sequence data
https://case-group.github.io/
GNU General Public License v3.0
39 stars 17 forks source link

Keep alignment file #50

Closed hoelzer closed 3 years ago

hoelzer commented 3 years ago

For a more detailed variant calling QC it would be good as a first step to keep the alignment file. Best would be to have PSL format (I think like minimap2 directly can provide it)

RaverJay commented 3 years ago

Keep in mind there are now two alignment files, for each sample:

hoelzer commented 3 years ago

Good point.

For actual variant QC of the reconstructed consensus it would be good to publish the

It is also debatable how much sense it makes to do such a variant QC in a similar way then for illumina, but we will investigate this at some point and thus it would be good to have the BAMs.

(actually... the BAMs are already in the 2.Genomes folder I think... so maybe this is alreay fine and we can close my request)

replikation commented 3 years ago

yep the bams for read against the genome (not wuhan) are in the genome folder