I've been utilizing the imgt.202312-3.sv8.json database with the latest MiXCR for extracting heavy and light chains from dog species. While I've successfully extracted heavy and light-lambda chains, I encountered issues with light-kappa chains. Specifically, of the 10 samples, only one produced a light-kappa file, containing a minimal number of sequences (one or two), which seems like an artifact. Given that some of the sequenced samples are known to contain kappa chains, this result is unexpected.
I'm looking for the culprit behind the lack of kappa hains. Any advice would be appreciated!
Hi,
I've been utilizing the imgt.202312-3.sv8.json database with the latest MiXCR for extracting heavy and light chains from dog species. While I've successfully extracted heavy and light-lambda chains, I encountered issues with light-kappa chains. Specifically, of the 10 samples, only one produced a light-kappa file, containing a minimal number of sequences (one or two), which seems like an artifact. Given that some of the sequenced samples are known to contain kappa chains, this result is unexpected.
I'm looking for the culprit behind the lack of kappa hains. Any advice would be appreciated!