rezakj / iCellR

Single (i) Cell R package (iCellR) is an interactive R package to work with high-throughput single cell sequencing technologies (i.e scRNA-seq, scVDJ-seq, scATAC-seq, CITE-Seq and Spatial Transcriptomics (ST)).

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load10x failed #13

Closed jajcobyang closed 4 years ago

jajcobyang commented 4 years ago

Hello,

I am trying to test out iCellR, however, I can't load my 10x data. rm(list = ls()) options(stringsAsFactors = F)

library(iCellR)

my.data <- load10x(dir.10x ='../reads' )

Error: cannot allocate vector of size 6288.3 Gb

` In reads, there 3 files :

Thanks!

rezakj commented 4 years ago

How many barcodes there are and how big those files are? It seems like it's memory issue.