I like the way you begin each section with its own little behind the scenes thought process blurb, makes it easy to follow as a reader.
• You are the first code I have read that reverse compliments the UMI based on strandedness! I have this in my code, but after reading more comments and pseudocode it may or may not be necessary?
• Your preprocessing, sorting out unmapped and secondary alignments, is quite smart.
• You may need to be tallying removed duplicates, we will probably need to do some data output on how many were removed.
• I would mention you don’t seem to write the header lines to the final file, but the first reviewers caught that already.
I like the way you begin each section with its own little behind the scenes thought process blurb, makes it easy to follow as a reader. • You are the first code I have read that reverse compliments the UMI based on strandedness! I have this in my code, but after reading more comments and pseudocode it may or may not be necessary? • Your preprocessing, sorting out unmapped and secondary alignments, is quite smart. • You may need to be tallying removed duplicates, we will probably need to do some data output on how many were removed. • I would mention you don’t seem to write the header lines to the final file, but the first reviewers caught that already.