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rnabioco / clustifyr-web-app

An R Shiny app to help with scRNA-seq benchmarking and analysis with clustifyr
https://raysinensis.shinyapps.io/clustifyr-web-app/
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Handling normalized matrix #17

Closed raysinensis closed 3 years ago

raysinensis commented 3 years ago

Adopt the checking code in clustifyr you already wrote Essentially, skip normalization in the seurat object creation process

UMIMatrix <- data1()
matrixSeuratObject <- CreateSeuratObject(counts = UMIMatrix, project = "Seurat object matrix", min.cells = 0, min.features = 0)
matrixSeuratObject <- FindVariableFeatures(matrixSeuratObject, selection.method = "vst", nfeatures = 2000)`