I am running into an issue with using SangerAlignment() on some of my .ab1 files. I have used it in the past with no problem, but for files from my last two batches of sequencing, I run into the following error code:
Error in .local(.Object, ...) :
'qualityPhredScores' length cannot be zero.PARAMETER_VALUE_ERROR
In addition: Warning message:
In read.abif(readFileName) : unimplemented legacy type found in file
I can't seem to identify the difference between the .ab1 files that is triggering the error.
Hello,
I am running into an issue with using SangerAlignment() on some of my .ab1 files. I have used it in the past with no problem, but for files from my last two batches of sequencing, I run into the following error code:
Error in .local(.Object, ...) : 'qualityPhredScores' length cannot be zero.PARAMETER_VALUE_ERROR In addition: Warning message: In read.abif(readFileName) : unimplemented legacy type found in file
I can't seem to identify the difference between the .ab1 files that is triggering the error.
Thanks! Cody