Hi,
We like to automate our variant calling proces from targeted sanger sequences of genes that our associated to resistance by mapping to a WT (susceptible) reference. Ideally we would like to have a codon based annotation of the variant in case of synonymous or nonsynonymous mutations and nucleotide position in case of indels. (E.g. nonsynonymous Arg60Lys(aac>aag) or other format). Based on the documentation, I assume this is not integrated in to your tool. Could you advise?
Best,
wim
Hi, We like to automate our variant calling proces from targeted sanger sequences of genes that our associated to resistance by mapping to a WT (susceptible) reference. Ideally we would like to have a codon based annotation of the variant in case of synonymous or nonsynonymous mutations and nucleotide position in case of indels. (E.g. nonsynonymous Arg60Lys(aac>aag) or other format). Based on the documentation, I assume this is not integrated in to your tool. Could you advise? Best, wim