Hi!
I have sequencing data that I processed with sangeranalyseR and it did a great job (thank you for providing this tool!). Another part of my dataset, however, is only available in the SCF format. I tried to convert it to ab1 through a couple of tools (i.e. Geneious and staden) but sangeranalyser can't read their output files (e.g. I got error messages stating problems due to either "incorrect format" or "unimplemented legacy found in file"). Since sangeranalyseR makes use of the sangerseqR "read.abif" tool for reading ab1 files, I was wondering if perhaps the equivalent tool in sangerseqR for reading SCF files could also be added to sangeranalyseR? If I can load SCF files into a sangeranalyseR object, then I can use the same steps that I used previously for my ab1 data. Alternatively, I was wondering if you would have any advice on how to convert scf to ab1, or perhaps on how to use sangerseqR to load my SCF data into R and then import it to sangeranalyseR.
Hi! I have sequencing data that I processed with sangeranalyseR and it did a great job (thank you for providing this tool!). Another part of my dataset, however, is only available in the SCF format. I tried to convert it to ab1 through a couple of tools (i.e. Geneious and staden) but sangeranalyser can't read their output files (e.g. I got error messages stating problems due to either "incorrect format" or "unimplemented legacy found in file"). Since sangeranalyseR makes use of the sangerseqR "read.abif" tool for reading ab1 files, I was wondering if perhaps the equivalent tool in sangerseqR for reading SCF files could also be added to sangeranalyseR? If I can load SCF files into a sangeranalyseR object, then I can use the same steps that I used previously for my ab1 data. Alternatively, I was wondering if you would have any advice on how to convert scf to ab1, or perhaps on how to use sangerseqR to load my SCF data into R and then import it to sangeranalyseR.