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ropensci / tidyqpcr

quantitative PCR analysis in the tidyverse
https://docs.ropensci.org/tidyqpcr/
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Plate functions for split-loading with standard multichannel pipettes #25

Open ewallace opened 4 years ago

ewallace commented 4 years ago

So far the create_plate helper functions assume that plates are in 8x8 (or 6x8, 4x4) tight blocks. This is fine for 96-well plates with standard multichannel pipettes, or for 384-well plates with variable width pipettes. However loading 384-well plates from a 96-well plate or strip with fixed-width multichannel pipettes results in loading every 2nd well with one pipette run. This could result in adjacent wells being "paired" if we move the multichannel 1 across for a duplicate. Or adjacent wells being unrelated if two entirely different approaches are taken.

First step is to ask for some example plate layouts from real users, then figure out what is needed.

Related to #20 and #2.

ewallace commented 4 years ago

Discussed as a hint in platesetup_vignette.Rmd, as updated in #37.

ewallace commented 4 years ago

We are waiting to address this until a user asks for it and provides some example plate layouts and data.

Might be addressable with plater, related to #12.