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ropensci / tidyqpcr

quantitative PCR analysis in the tidyverse
https://docs.ropensci.org/tidyqpcr/
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Create new tidyqpcr vignette with MIQE compliant raw data #73

Open DimmestP opened 4 years ago

DimmestP commented 4 years ago

The current delta-cq vignette shows how different forms of qPCR data can be incorporated and analysed by tidyqpcr but is not a good example of plate design according to the tidyqpcr ethos. A new vignette going from raw qpcr machine data to publishable results with MIQE compliant, tidy plate design would be a perfect stepping stone for new users.

ewallace commented 4 years ago

I agree this would be good. Let's design an experiment for this, just 2 conditions, 3 tech reps, 2 bio reps (or more), correct controls, that fits on a 96-well plate.

2 conditions (3 tech reps + -RT 1 tech rep ) 3 biol reps * 4 targets = 96 wells

ewallace commented 4 years ago

Reminder that we should design an experiment for this. We might even be able to ask @j-aux really nicely and she might do that experiment.

ewallace commented 4 years ago

There is a lot of old RNA extracted from Heat-shocked yeast (by me in 2018) in the -80 freezer. If that RNA is still ok, then we could use it. Or do a fresh "quick" experiment.