In creating the reduced genome it is necessary to specify the fragment length as length of the read - (size of barcode+size of primer including the RE sequence). However, in our NextGen sequencing the barcode indexes are automatically trimmed during demultiplexing. Does that mean that I should only subtract the length of the primer sequence and the RE? Thanks!
rr1859
commented
7 years ago
Hi,
In creating the reduced genome it is necessary to specify the fragment length as length of the read - (size of barcode+size of primer including the RE sequence). However, in our NextGen sequencing the barcode indexes are automatically trimmed during demultiplexing. Does that mean that I should only subtract the length of the primer sequence and the RE? Thanks!