In step 3. Removing the self-ligated and undigested fragments. You use the command:
grep -i -B 1 "CTTCTATCTGCAGAGA" mm10_hindiii_flanking_sequences_25_unique_2.fa
Is this bait string "CTTCTATCTGCAGAGA" for example dataset "CD83"? I find the bait string for your example dataset different, "CTAGTTAGTCATGG". Which data set is "CTTCTATCTGCAGAGA" for then?
Hi,
In step 3. Removing the self-ligated and undigested fragments. You use the command: grep -i -B 1 "CTTCTATCTGCAGAGA" mm10_hindiii_flanking_sequences_25_unique_2.fa
Is this bait string "CTTCTATCTGCAGAGA" for example dataset "CD83"? I find the bait string for your example dataset different, "CTAGTTAGTCATGG". Which data set is "CTTCTATCTGCAGAGA" for then?
Thanks!