I performed the full 4C-seq analysis using the R.4Cker package and I am having some comprehension problems with the outputs.
Regarding the out_trans_norm_counts.bedgraph output, what is the last two columns? And what are they normalized to? Is this value in RPM?
Further, by summing the lines in the output files out_trans_highinter.bed, out_trans_lowinter.bed and out_trans_noninter.bed, we obtain 1091. However the output file out_trans_norm_counts.bedgraph has 3647 lines. How can this happen?
Thank you for your gentle attention and all the work
Hi!
I performed the full 4C-seq analysis using the R.4Cker package and I am having some comprehension problems with the outputs. Regarding the out_trans_norm_counts.bedgraph output, what is the last two columns? And what are they normalized to? Is this value in RPM? Further, by summing the lines in the output files out_trans_highinter.bed, out_trans_lowinter.bed and out_trans_noninter.bed, we obtain 1091. However the output file out_trans_norm_counts.bedgraph has 3647 lines. How can this happen? Thank you for your gentle attention and all the work
Cheers, R