Custom adapters cannot be found when binning but can be found using trimming function

[v1tazhan9]

Hi! I input custom adapter using adapter.py, these adapters can be found if i use -o function for trimming

"30,139 / 32,299 reads had adapters trimmed from their end (2,444,748 bp removed)"

but when I use - b function to bin, and it says "Error: no barcodes were found, so Porechop cannot perform barcode demultiplexing" i try to set the threshold really really low just to try and get nothing, tried verbosity for debugging and nothing

here's a history: % porechop -i /Users/vita/Desktop/GladLab/Nanopore_DEPC_data/R1_A_probed/fastq_pass/R1_A_probed.fastq -b /Users/vita/Desktop/GladLab/Nanopore_DEPC_data/porechop/bin --barcode_threshold 20 --barcode_diff 1 --verbosity 3

Loading reads /Users/vita/Desktop/GladLab/Nanopore_DEPC_data/R1_A_probed/fastq_pass/R1_A_probed.fastq 28,518 reads loaded

Looking for known adapter sets 10,000 / 10,000 (100.0%) Error: no barcodes were found, so Porechop cannot perform barcode demultiplexing

[fernanarr]

Same problem for me.

I've also tried executing porechop from its directory with python3 porechop-runner.py with the same result.

-o option finds custom barcodes, but -b can't find none of them.