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rrwick / Porechop

adapter trimmer for Oxford Nanopore reads
GNU General Public License v3.0
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updated adapter sequences #32

Closed jvolkening closed 6 years ago

jvolkening commented 6 years ago

This is the adapters.py we have modified to work with our libraries. However, these are based on the standard ONT PCR-based barcoding kit, and as such I thought they might be useful in the main branch. The added primer sequences share some overlap with existing adapters in the file, but are not identical. The full adapter sequences in our 1D libraries look like this (or the reverse complement):

5'-universal -> barcode -> pcr_tail_1 -> insert <- pcr_tail_2 <- barcode <- universal-3'

The universal adapter seems to be identical to the existing SQK-NSK007.

rrwick commented 6 years ago

Thanks! I've merged it into Porechop's development branch, and it should make it into the next release.