Lambda SQK-NSK007 and crossbarcoding detection

[davidvilanova]

Hi, I´m running porechop and used multiplex outpu on one sample that was built with barcode01 only.

Here below the results.

From the image below it looks that porechop is checking for lambda DNA. The green colors means it was found ?? How can i check if lambda DNA was found ??

I also see that other barcodes were match although the sample was prepared with only barcode01. Are those mismatches or contaminations ?

Thanks !!!!

[Huanle]

Hi @davidvilanova ,

have you sorted it out?

This supports your guess that green denotes matches.

[davidvilanova]

Hi @Huanle , i end up using guppy which now incorporates demultiplexing and barcode trimming.

Best,

[Huanle]

Hi @davidvilanova ,

I also rely on guppy for demultiplexing. But I am not sure if guppy also trims adapters though it has relevant flags that seem to be adjustable for adapter identification. Do you happen to have any idea about that? Thanks a lot.

[davidvilanova]

Yes it trims barcodes

[Huanle]

But it does not say whether it trims adapters :-)

[davidvilanova]

You have some options to define that

[mzakram219]

Hi @davidvilanova, could you please provide some information, how can i use command base guppy for demultiplexing and adapter trimming? I tried my best to find guppy .exe files, but i do not have any idea where to get it.