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rrwick / Porechop

adapter trimmer for Oxford Nanopore reads
GNU General Public License v3.0
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Custom barcodes not found #94

Open termithorbor opened 1 year ago

termithorbor commented 1 year ago

Hi,

I have designed a custom dual indexing approach based on the ONT Native barcoding primers:

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Ont_flank1 forward | Barcode forward | Ont_flank2 forward -- | -- | -- AAGGTTAA | CACAAAGACACCGACAACTTTCTT | CAGCACCT AAGGTTAA | ACAGACGACTACAAACGGAATCGA | CAGCACCT AAGGTTAA | CCTGGTAACTGGGACACAAGACTC | CAGCACCT

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Ont_flank1 reverse | Barcode reverse | Ont_flank2 reverse -- | -- | -- GGTGCTG | AAGAAAGTTGTCGGTGTCTTTGTG | TTAACCTTAGCAAT GGTGCTG | TCGATTCCGTTTGTAGTCGTCTGT | TTAACCTTAGCAAT GGTGCTG | GAGTCTTGTGTCCCAGTTACCAGG | TTAACCTTAGCAAT

My actual barcodes are surrounded by the ONT flanks. I basically combine a forward and a reverse primer resulting in a unique primer-pair. Is there a way to achive demultiplexing with porechop?

Thanks in advance.

Dafnaa commented 1 year ago

Hii, I'm trying the same thing, have you found a solution yet?