Multiplicity is the number of times a sequence occurs in the underlying sequence. Single-copy contigs (those with a multiplicity of one, occurring only once in the underlying sequence) are particularly use
full"
I used the following command:
/home/bin/Unicycler-master/unicycler-runner.py --short1 /home/1.fastq.gz --short2 /home/2.fastq.gz --long /home/ONT.fastq.gz
--out han_unicyc --spades_path /home/bin/spades-3.13.0/assembler/bin/spades.py --makeblastdb_path /home/bin/ncbi-blast-2.11.0+/bin/makeblastdb --tblastn_path /home/bin/ncbi-blast-2.11.0+/bin/
tblastn --racon_path /home/bin/racon/build/bin/racon --bowtie2_path /home/bin/bowtie2-2.2.4/bowtie2 --bowtie2_build_path /home/bin/bowtie2-2.2.4/bowtie2-build --samtools_path /home/bin/
samtools-1.11/samtools --pilon_path /home/bin/pilon-1.23.jar --threads 40 --mode bold
Hi, I am trying to assemble the plant mitochondrial genome using both Illumina and ONT reads. I am getting an error
"Determining graph multiplicity (2022-07-01 04:59:06)
full"
I used the following command: /home/bin/Unicycler-master/unicycler-runner.py --short1 /home/1.fastq.gz --short2 /home/2.fastq.gz --long /home/ONT.fastq.gz --out han_unicyc --spades_path /home/bin/spades-3.13.0/assembler/bin/spades.py --makeblastdb_path /home/bin/ncbi-blast-2.11.0+/bin/makeblastdb --tblastn_path /home/bin/ncbi-blast-2.11.0+/bin/ tblastn --racon_path /home/bin/racon/build/bin/racon --bowtie2_path /home/bin/bowtie2-2.2.4/bowtie2 --bowtie2_build_path /home/bin/bowtie2-2.2.4/bowtie2-build --samtools_path /home/bin/ samtools-1.11/samtools --pilon_path /home/bin/pilon-1.23.jar --threads 40 --mode bold
Thank you.