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ruanjue / smartdenovo

Ultra-fast de novo assembler using long noisy reads
GNU General Public License v3.0
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separating similar chromosome parts in corrected read assemblies #27

Closed chklopp closed 5 years ago

chklopp commented 5 years ago

We are assembling a large genome (close to 2 Gb). We have corrected the reads with canu and assembled them with smartdenovo. The assembly size is 16% lower than expected. Some chromosomes share large sub-sequences which are assembled only once and collected twice the average read depth when we realign the reads on the assembly. We think that these parts have not different enough sequences to be separated by smartdenovo with standard parameters. Have you already seen this? Which parameter(s) should we change to try to separate both parts?

ruanjue commented 5 years ago

-m in wtzmo may help. Please note that I am maintaining wtdbg2 instead of smartdenovo, please try wtdbg2.