ruanjue / smartdenovo

Ultra-fast de novo assembler using long noisy reads
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make: *** [JBX.dmo.lay] Error 1 #58

Open zxgsy520 opened 1 year ago

zxgsy520 commented 1 year ago

Hi! I assembled a genome of about 7G and reported the following error. Ask if it is resolved. image thanks in advance!

ruanjue commented 1 year ago

This problem was caused by so looong reads now comparing to that at 2014 year. I used 20 bits (1Mb) to store the overlap length, which was thought to be safe, but not.

1050571 - (1 ^ 20) = 1995

You know, change the data structure will cause unexcepted errors, esp. for this eight year ago programs. So, my solution will be split any longer read into parts no longer than 1Mb. If 1Mb is still to long for other part of whole pipeline, try 256kb.

Hope it helps.