search

ruanjue / wtdbg2

Redbean: A fuzzy Bruijn graph approach to long noisy reads assembly
GNU General Public License v3.0
504 stars 92 forks source link

Ambiguous bases #254

Open OliverPStuart opened 1 year ago

OliverPStuart commented 1 year ago

Hi there,

I am examining a wtdbg2.5 assembly and I have two contigs with short (23 bp) stretches of ambiguous N bases. This is not a problem per se but I do not understand what these Ns represent.

Can you please advise?

Best, Ollie

ruanjue commented 1 year ago

Possiblly caused by mis-joint of seqs of nearby edges, but this bug is too rare to be debuged.