search

rvolden / 10xR2C2

Scripts for analyzing 10x R2C2 data
MIT License
6 stars 6 forks source link

results of 10xR2C2 #4

Open ssscj opened 3 years ago

ssscj commented 3 years ago

Hi, I got results of the MergeUMIs10x.py, which consisted of .matched_reads.txt, .merged.fasta, .merged.subreads.fastq and .UMI_only.fasta. What is the difference between .UMI_only.fasta and .merged.fasta ? And merged.fasta contains more reads than UMI_only.fasta. So which one should I use for analysis? Thank you.

Best regards, Chujie

rvolden commented 3 years ago

The UMI_only.fasta file contains only the reads that had a matching UMI and got re-consensus called. For analysis, you'll want to use the merged.fasta file since those will contain both the UMI reads and the non-UMI merged reads.