Hello,
Thanks for the amazing tool.
I'm trying to analyze the long read data generated from the ONT sequencer according to your C3POa work flow, but the pre-processing doesn't continue and finished at Calling consensi.
Despite the tools were installed with their dependencies, and I prepared the UMI_Splint.fasta used in the experiment, but unfortunately the process stopped as showed below:
Log Contents:
$ cat/out/c3poa.log
C3POa version: v2.2.3
Total reads: 1687451
No splint reads: 1505291 (89.21%)
Under len cutoff: 15 (0.00%)
Total thrown away reads: 1505306 (89.21%)
Reads after preprocessing: 182145
Could you please help me to figure out what is the problem?
Hello, Thanks for the amazing tool. I'm trying to analyze the long read data generated from the ONT sequencer according to your C3POa work flow, but the pre-processing doesn't continue and finished at Calling consensi. Despite the tools were installed with their dependencies, and I prepared the UMI_Splint.fasta used in the experiment, but unfortunately the process stopped as showed below:
command: (base) [ukhussein@ldragon3 C3POa-2.2.3]$ python3 C3POa.py -r ../../projects/nanopore_R2C2/10X_071_R2C2/test/dngqu0264_71_fastq_pass.tar.gz -s ./UMI_Splint.fasta/UMI_Splints.fasta -d 500 -l 100 -g 1000 -n 32 -o out2
Output:
Log Contents: $ cat/out/c3poa.log C3POa version: v2.2.3 Total reads: 1687451 No splint reads: 1505291 (89.21%) Under len cutoff: 15 (0.00%) Total thrown away reads: 1505306 (89.21%) Reads after preprocessing: 182145
Could you please help me to figure out what is the problem?