in the tutorial "04_TranscriptionFactor_activity_with_Dorothea", the input file for differential expression analysis had a normalized enrichment score (NES). I performed my differential expression using seurat FindMarkers function on scRNA data, therefore I didn't have an NES in my results, now I want to use this list of DEGs for transcription factors analysis in Dorothea, what parameters should I include in the function dorothea::run_viper. In other words, how to calculate NES from Log2FC or run the analysis directly using log2FC or FDR.
in the tutorial "04_TranscriptionFactor_activity_with_Dorothea", the input file for differential expression analysis had a normalized enrichment score (NES). I performed my differential expression using seurat FindMarkers function on scRNA data, therefore I didn't have an NES in my results, now I want to use this list of DEGs for transcription factors analysis in Dorothea, what parameters should I include in the function dorothea::run_viper. In other words, how to calculate NES from Log2FC or run the analysis directly using log2FC or FDR.