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I was wondering if bcftools might have a feature I might be unaware of that I've used with other variant callers. I am comparing vcfs in polyploid individuals with various average read depths, and I fear some sites may be getting called incorrectly just due to chance if they are low depth. E.g., for a tetraploid, sites with AABB are more likely to get correctly called as heterozygous compared to sites that are AAAB, because each allele has 50% chance of being represented for every read sampled in the former scenario.
Without going into too much detail, I'd like to focus on sites that have a better chance of showing up correctly in low coverage data; I'm comparing a mother (higher coverage) to a child (lower coverage) where the father is unknown, and trying to see if sites match. I can provide more details if I'm being too vague or if this is confusing, but I didn't want this issue to be too long.
Is there an easy way to filter on the fraction of reads supporting each allele? A flag I might be unaware of that would make this easier?
Hi there,
I was wondering if bcftools might have a feature I might be unaware of that I've used with other variant callers. I am comparing vcfs in polyploid individuals with various average read depths, and I fear some sites may be getting called incorrectly just due to chance if they are low depth. E.g., for a tetraploid, sites with AABB are more likely to get correctly called as heterozygous compared to sites that are AAAB, because each allele has 50% chance of being represented for every read sampled in the former scenario.
Without going into too much detail, I'd like to focus on sites that have a better chance of showing up correctly in low coverage data; I'm comparing a mother (higher coverage) to a child (lower coverage) where the father is unknown, and trying to see if sites match. I can provide more details if I'm being too vague or if this is confusing, but I didn't want this issue to be too long.
Is there an easy way to filter on the fraction of reads supporting each allele? A flag I might be unaware of that would make this easier?
Thanks so much for your time! Charity