Open kubu4 opened 4 years ago
Wow - that error used to happen years ago when you used a Genbank reference. But it works with GFF 3.0 normally.
Did you run art ref.fa + ref.gff
?
One idea might be to make a single GFF file as follows:
echo "##gff-version 3" > fixed.gff
cat ref.gff >> fixed.gff
echo "##FASTA" >> fixed.gff
cat ref.fa >> fixed.gff
and try loading that
I have a multi-FastA file containing 18 genomic scaffolds. I also have a GFF containing gene features for all of the scaffolds. However, Artemis appears to be mapping all gene features to each and every scaffold.
The screencap below shows:
Green box highlights different scaffold names in the gene GFF
Red arrow shows Artemis currently viewing "Scaffold_01"
Green arrows show gene GFF coordinates from different scaffolds are listed in the Artemis
Scaffold_01
features list, even though only one of the gene features actually belongs inScaffold_01
Any thoughts on what is happening here and/or how to correct this?