_clustered.clstr file does not exist, cannot be read

[Haley-Wami]

I am using the latest version of Roary (v3.13.0) with cdhit 4.8. But I still get this issue "_clustered.clstr file does not exist, cannot be read" and the warning "perl: warning: Falling back to the standard locale ("C")"

perl: warning: Falling back to the standard locale ("C"). Cant open file: _clustered.clstr ------------- EXCEPTION: Bio::Root::Exception ------------- MSG: Could not read file '_clustered': No such file or directory STACK: Error::throw STACK: Bio::Root::Root::throw /usr/local/share/perl/5.22.1/Bio/Root/Root.pm:445 STACK: Bio::Root::IO::_initialize_io /usr/local/share/perl/5.22.1/Bio/Root/IO.pm:268 STACK: Bio::SeqIO::_initialize /usr/local/share/perl/5.22.1/Bio/SeqIO.pm:508 STACK: Bio::SeqIO::fasta::_initialize /usr/local/share/perl/5.22.1/Bio/SeqIO/fasta.pm:88 STACK: Bio::SeqIO::new /usr/local/share/perl/5.22.1/Bio/SeqIO.pm:384 STACK: Bio::SeqIO::new /usr/local/share/perl/5.22.1/Bio/SeqIO.pm:430 STACK: Bio::Roary::ChunkFastaFile::_input_seqio /usr/local/share/perl/5.22.1/Bio/Roary/ChunkFastaFile.pm:27 STACK: Bio::Roary::ChunkFastaFile::sequence_file_names /usr/local/share/perl/5.22.1/Bio/Roary/ChunkFastaFile.pm:48 STACK: Bio::Roary::ParallelAllAgainstAllBlast::_sequence_file_names /usr/local/share/perl/5.22.1/Bio/Roary/ParallelAllAgainstAllBlast.pm:61 STACK: Bio::Roary::ParallelAllAgainstAllBlast::run /usr/local/share/perl/5.22.1/Bio/Roary/ParallelAllAgainstAllBlast.pm:112 STACK: Bio::Roary::run /usr/local/share/perl/5.22.1/Bio/Roary.pm:94 STACK: Bio::Roary::CommandLine::Roary::run /usr/local/share/perl/5.22.1/Bio/Roary/CommandLine/Roary.pm:325 STACK: /mnt/WD5/opt/roary/Roary-3.13.0/bin/roary:19

Any ideas on what to do? Thanks in advance!

[chen202101]

I have the same problem, have you solved it？

[Haley-Wami]

@chen202101 unfortunately not, used genapi (alternative tool) instead.