sanger-pathogens / circlator

A tool to circularize genome assemblies
http://sanger-pathogens.github.io/circlator/
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Failed to Partition the reads #147

Open Frazapel opened 5 years ago

Frazapel commented 5 years ago

I run circlator with this command:

circlator all --assembler canu --merge_min_id 85 --merge_breaklen 1000 --threads 24 final.genome.scf.fasta canu_correct/Kleb_canu_correct.correctedReads.fasta.gz circlator

using Nanopore reads, our assembly was made on masurca using nanopore and illumina reads.

This is the error I got:

ERROR: ERROR: Failed with exit code 1. (rc=256) ERROR:

Don't panic, but a mostly harmless error occurred and canu failed.

Disk space available: 179.056 GB

Last 50 lines of the relevant log file (/home/user5/Task_II/II.MinION_multiplex_Klebsiellapneumoniae/CA.mr.41.15.15.0.02/circlator/TESTlator/03.assemble/unitigging/canu.utgStore/partitionedReads.err):

tgStore::tgStore()-- ERROR, didn't find any tigs in the store. tgStore::tgStore()-- asked for store '/home/user5/Task_II/II.MinION_multiplex_Klebsiellapneumoniae/CA.mr.41.15.15.0.02/circlator/TESTlator/03.assemble/unitigging/canu.utgStore', correct? tgStore::tgStore()-- asked for version '1', correct?

canu failed with 'failed to partition the reads'.

Can you help me with this?