search

sanger-pathogens / circlator

A tool to circularize genome assemblies
http://sanger-pathogens.github.io/circlator/
Other
221 stars 56 forks source link

After running circlator reduction of 6 genomes in assembly to 3. #150

Open severst opened 5 years ago

severst commented 5 years ago

Hi, I am using Rolling Circle Amplification technique which produces very long amplicons. After amplification, I fragment these products to approximately 1 genomelength and sequence this by MinION. I assembled the data with Canu, which ends up in an assembly of 6 times the genome length, since there were multiple reads >2 times the total length. By using Circlator, I was able to reduce the assembly length to 3 times the genome. Is it possible to reduce this to 1? Or do I have to manually cut my reads to succeed? Thanks, Tim