I used the canu + Polish + circlator to assembly bacterial genomes.
The largest contig in canu assembly is considered as 'suggestCircular'.
But in the circlator :
[merge circularise_details] tig00000001|arrow Checking 8 nucmer hits
[merge circularise_details] tig00000001|arrow potential pair of nucmer hits for circularization:
[merge circularise_details] tig00000001|arrow 1 12322 29044 41367 12322 12324 97.11 4417207 76689 1 tig00000001|arrow NODE_4_length_76689_cov_22.460131
[merge circularise_details] tig00000001|arrow 4367209 4408566 1 41367 41358 41367 99.17 4417207 76689 1 tig00000001|arrow NODE_4_length_76689_cov_22.460131
[merge circularise_details] tig00000001|arrow cannot use this pair because longer match was found
[merge circularise_details] tig00000001|arrow cannot use this pair because positions/orientations of matches no good
[merge circularise_details] tig00000001|arrow Trying to circularize. Has nucmer hits to check...
[merge circularise_details] tig00000001|arrow No matches to SPAdes circular contigs
[merge circularise_details] tig00000001|arrow Could not circularize using matches to spades circular contigs
[merge circularise_details] tig00000001|arrow Cannot circularize: no suitable nucmer hits
[merge circularise_details] tig00000001|arrow Circularized: no
How can I optimize the parameters for circlator to consider this contig as Circularized?
Hello,
I used the canu + Polish + circlator to assembly bacterial genomes. The largest contig in canu assembly is considered as 'suggestCircular'. But in the circlator :
[merge circularise_details] tig00000001|arrow Checking 8 nucmer hits [merge circularise_details] tig00000001|arrow potential pair of nucmer hits for circularization: [merge circularise_details] tig00000001|arrow 1 12322 29044 41367 12322 12324 97.11 4417207 76689 1 tig00000001|arrow NODE_4_length_76689_cov_22.460131 [merge circularise_details] tig00000001|arrow 4367209 4408566 1 41367 41358 41367 99.17 4417207 76689 1 tig00000001|arrow NODE_4_length_76689_cov_22.460131 [merge circularise_details] tig00000001|arrow cannot use this pair because longer match was found [merge circularise_details] tig00000001|arrow cannot use this pair because positions/orientations of matches no good [merge circularise_details] tig00000001|arrow Trying to circularize. Has nucmer hits to check... [merge circularise_details] tig00000001|arrow No matches to SPAdes circular contigs [merge circularise_details] tig00000001|arrow Could not circularize using matches to spades circular contigs [merge circularise_details] tig00000001|arrow Cannot circularize: no suitable nucmer hits [merge circularise_details] tig00000001|arrow Circularized: no
How can I optimize the parameters for circlator to consider this contig as Circularized?
Thanks!!!!