Hello, I'm trying to troubleshoot why my mitochondrial contig wasn't circularized, and in 03.assemble>warnings.log I found
* 0:00:03.219 15M / 74M WARN General (launcher.cpp : 172) Your data seems to have high uniform coverage depth. It is strongly recommended to use --isolate option.
Therefore I tried adding --assemble_spades_isolate, but get circlator: error: unrecognized arguments: --assemble_spades_isolate.
Is there a different way the isolate option should be added?
Hello, I'm trying to troubleshoot why my mitochondrial contig wasn't circularized, and in 03.assemble>warnings.log I found
* 0:00:03.219 15M / 74M WARN General (launcher.cpp : 172) Your data seems to have high uniform coverage depth. It is strongly recommended to use --isolate option.Therefore I tried adding
--assemble_spades_isolate, but getcirclator: error: unrecognized arguments: --assemble_spades_isolate.Is there a different way the isolate option should be added?
Best regards, Brett