Hello, I am trying to run circlator using canu (v 1.9)as the assembler
circlator all --assembler canu ~/Desktop/CANU/KU15176/assembly/KU15176.contigs.fasta ~/Desktop/CANU/KU15176/corrected_KU15176/KU15176.correctedReads.fasta.gz ~/Desktop/Circulator_KU15176CANU
however the run gets terminated and I am getting the following error:
Traceback (most recent call last):
File "/usr/bin/circlator", line 58, in
exec('circlator.tasks.' + task + '.run()')
File "", line 1, in
File "/home/rem/.local/lib/python3.8/site-packages/circlator/tasks/all.py", line 169, in run
a.run()
File "/home/rem/.local/lib/python3.8/site-packages/circlator/assemble.py", line 179, in run
self.run_canu()
File "/home/rem/.local/lib/python3.8/site-packages/circlator/assemble.py", line 172, in run_canu
os.rename(original_gfa, renamed_gfa)
FileNotFoundError: [Errno 2] No such file or directory: '/home/rem/Desktop/Circulator_KU15176CANU/03.assemble/canu.contigs.gfa' -> '/home/rem/Desktop/Circulator_KU15176CANU/03.assemble/contigs.gfa'
Can anybody help me on this? your assistance will be greatly appreciated.
Thank you very much!
Now that contigs.gfa got removed. The contigs.gfa file has not been generated sinceCanu v1.9, downgrading canu should fix this error. see circular contigs and contigs.gfa #1551
Hello, I am trying to run circlator using canu (v 1.9)as the assembler
circlator all --assembler canu ~/Desktop/CANU/KU15176/assembly/KU15176.contigs.fasta ~/Desktop/CANU/KU15176/corrected_KU15176/KU15176.correctedReads.fasta.gz ~/Desktop/Circulator_KU15176CANU
however the run gets terminated and I am getting the following error: Traceback (most recent call last): File "/usr/bin/circlator", line 58, in
exec('circlator.tasks.' + task + '.run()')
File "", line 1, in
File "/home/rem/.local/lib/python3.8/site-packages/circlator/tasks/all.py", line 169, in run
a.run()
File "/home/rem/.local/lib/python3.8/site-packages/circlator/assemble.py", line 179, in run
self.run_canu()
File "/home/rem/.local/lib/python3.8/site-packages/circlator/assemble.py", line 172, in run_canu
os.rename(original_gfa, renamed_gfa)
FileNotFoundError: [Errno 2] No such file or directory: '/home/rem/Desktop/Circulator_KU15176CANU/03.assemble/canu.contigs.gfa' -> '/home/rem/Desktop/Circulator_KU15176CANU/03.assemble/contigs.gfa'
Can anybody help me on this? your assistance will be greatly appreciated. Thank you very much!