I'm going to try looking into this, but in the meantime though I'd report it. Here's the output from my test run after following the documentation.
$ nextflow run companion -profile docker
N E X T F L O W ~ version 19.10.0
Launching companion/annot.nf [prickly_mcclintock] - revision: 63734f6d0c
C O M P A N I O N ~ version 1.0.2
query : /opt/companion/example-data/L_donovani.1.fasta
reference : LmjF.1
reference directory : /opt/companion/example-data/references
output directory : /opt/companion/example-output
[- ] process > truncate_input_headers -
[- ] process > truncate_input_headers -
[- ] process > sanitize_input -
executor > local (2)
[d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1
[- ] process > sanitize_input -
[- ] process > contiguate_pseudochromosomes -
[- ] process > predict_tRNA -
[4e/8398aa] process > press_ncRNA_cms [ 0%] 0 of 1
[- ] process > predict_ncRNA -
[- ] process > merge_ncrnas -
[- ] process > exonerate_empty_hints -
[- ] process > ratt_make_ref_embl -
[- ] process > run_ratt -
[- ] process > ratt_to_gff3 -
[- ] process > transcript_empty_hints -
[- ] process > merge_hints -
executor > local (2)
[d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1
[- ] process > sanitize_input -
executor > local (3)
[d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1
[- ] process > sanitize_input -
[- ] process > contiguate_pseudochromosomes -
[- ] process > predict_tRNA -
[4e/8398aa] process > press_ncRNA_cms [ 0%] 0 of 1
[- ] process > predict_ncRNA -
[- ] process > merge_ncrnas -
[e2/7fdb92] process > exonerate_empty_hints [ 0%] 0 of 1
[- ] process > ratt_make_ref_embl -
[- ] process > run_ratt -
[- ] process > ratt_to_gff3 -
[- ] process > transcript_empty_hints -
[- ] process > merge_hints -
[- ] process > run_augustus_pseudo -
[- ] process > run_augustus_contigs -
[- ] process > run_snap -
[- ] process > merge_genemodels -
[- ] process > integrate_genemodels -
[- ] process > fix_polycistrons -
[- ] process > remove_exons -
[- ] process > pseudogene_indexing -
[- ] process > pseudogene_last -
[- ] process > pseudogene_calling -
[- ] process > merge_structural -
[- ] process > add_gap_features -
executor > local (3)
[d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1
[- ] process > sanitize_input -
[- ] process > contiguate_pseudochromosomes -
[- ] process > predict_tRNA -
[4e/8398aa] process > press_ncRNA_cms [100%] 1 of 1, failed: 1 ✘
[- ] process > predict_ncRNA -
[- ] process > merge_ncrnas -
[e2/7fdb92] process > exonerate_empty_hints [ 0%] 0 of 1
[- ] process > ratt_make_ref_embl -
[- ] process > run_ratt -
[- ] process > ratt_to_gff3 -
[- ] process > transcript_empty_hints -
[- ] process > merge_hints -
[- ] process > run_augustus_pseudo -
[- ] process > run_augustus_contigs -
[- ] process > run_snap -
[- ] process > merge_genemodels -
[- ] process > integrate_genemodels -
[- ] process > fix_polycistrons -
[- ] process > remove_exons -
[- ] process > pseudogene_indexing -
[- ] process > pseudogene_last -
[- ] process > pseudogene_calling -
[- ] process > merge_structural -
[- ] process > add_gap_features -
executor > local (3)
[d4/a2f1c2] process > truncate_input_headers [100%] 1 of 1 ✔
[- ] process > sanitize_input -
[- ] process > contiguate_pseudochromosomes -
[- ] process > predict_tRNA -
[4e/8398aa] process > press_ncRNA_cms [100%] 1 of 1, failed: 1 ✘
[- ] process > predict_ncRNA -
[- ] process > merge_ncrnas -
[e2/7fdb92] process > exonerate_empty_hints [100%] 1 of 1, failed: 1
[- ] process > ratt_make_ref_embl -
[- ] process > run_ratt -
[- ] process > ratt_to_gff3 -
[- ] process > transcript_empty_hints -
[- ] process > merge_hints -
[- ] process > run_augustus_pseudo -
[- ] process > run_augustus_contigs -
[- ] process > run_snap -
[- ] process > merge_genemodels -
[- ] process > integrate_genemodels -
[- ] process > fix_polycistrons -
[- ] process > remove_exons -
[- ] process > pseudogene_indexing -
[- ] process > pseudogene_last -
[- ] process > pseudogene_calling -
[- ] process > merge_structural -
[- ] process > add_gap_features -
[- ] process > split_splice_models_at_gaps -
[- ] process > add_polypeptides -
[- ] process > get_proteins_for_orthomcl -
[- ] process > make_ref_input_for_orthomcl -
[- ] process > make_target_input_for_orthomcl -
[- ] process > blast_for_orthomcl_formatdb -
[- ] process > blast_for_orthomcl -
[- ] process > run_orthomcl -
[- ] process > annotate_orthologs -
[- ] process > run_pfam -
[- ] process > pfam_to_gff3 -
[- ] process > annotate_pfam -
[- ] process > make_distribution_gff -
[- ] process > make_distribution_gaf -
[- ] process > make_distribution_seqs -
[- ] process > make_genome_stats -
[- ] process > reference_compare -
[- ] process > make_tree -
[- ] process > blast_for_circos -
[- ] process > make_circos_inputs -
[- ] process > circos_run_chrs -
[- ] process > circos_run_bin -
[- ] process > merge_gff3_for_gff3toembl -
[- ] process > make_embl -
[- ] process > make_report -
[- ] process > make_genelist -
[- ] process > add_products_to_protein_fasta -
WARN: The operator first is useless when applied to a value channel which returns a single value by definition -- check channel ncrna_cmindex
WARN: Access to undefined parameter TRANSCRIPT_FILE -- Initialise it to a default value eg. params.TRANSCRIPT_FILE = some_value
WARN: The operator first is useless when applied to a value channel which returns a single value by definition -- check channel pseudochr_last_index
WARN: Killing pending tasks (1)
Error executing process > 'press_ncRNA_cms'
Caused by:
Process press_ncRNA_cms terminated with an error exit status (1)
I'm going to try looking into this, but in the meantime though I'd report it. Here's the output from my test run after following the documentation.
$ nextflow run companion -profile docker N E X T F L O W ~ version 19.10.0 Launching
companion/annot.nf
[prickly_mcclintock] - revision: 63734f6d0cC O M P A N I O N ~ version 1.0.2 query : /opt/companion/example-data/L_donovani.1.fasta reference : LmjF.1 reference directory : /opt/companion/example-data/references output directory : /opt/companion/example-output
[- ] process > truncate_input_headers - [- ] process > truncate_input_headers - [- ] process > sanitize_input - executor > local (2) [d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1 [- ] process > sanitize_input - [- ] process > contiguate_pseudochromosomes - [- ] process > predict_tRNA - [4e/8398aa] process > press_ncRNA_cms [ 0%] 0 of 1 [- ] process > predict_ncRNA - [- ] process > merge_ncrnas - [- ] process > exonerate_empty_hints - [- ] process > ratt_make_ref_embl - [- ] process > run_ratt - [- ] process > ratt_to_gff3 - [- ] process > transcript_empty_hints - [- ] process > merge_hints - executor > local (2) [d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1 [- ] process > sanitize_input - executor > local (3) [d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1 [- ] process > sanitize_input - [- ] process > contiguate_pseudochromosomes - [- ] process > predict_tRNA - [4e/8398aa] process > press_ncRNA_cms [ 0%] 0 of 1 [- ] process > predict_ncRNA - [- ] process > merge_ncrnas - [e2/7fdb92] process > exonerate_empty_hints [ 0%] 0 of 1 [- ] process > ratt_make_ref_embl - [- ] process > run_ratt - [- ] process > ratt_to_gff3 - [- ] process > transcript_empty_hints - [- ] process > merge_hints - [- ] process > run_augustus_pseudo - [- ] process > run_augustus_contigs - [- ] process > run_snap - [- ] process > merge_genemodels - [- ] process > integrate_genemodels - [- ] process > fix_polycistrons - [- ] process > remove_exons - [- ] process > pseudogene_indexing - [- ] process > pseudogene_last - [- ] process > pseudogene_calling - [- ] process > merge_structural - [- ] process > add_gap_features - executor > local (3) [d4/a2f1c2] process > truncate_input_headers [ 0%] 0 of 1 [- ] process > sanitize_input - [- ] process > contiguate_pseudochromosomes - [- ] process > predict_tRNA - [4e/8398aa] process > press_ncRNA_cms [100%] 1 of 1, failed: 1 ✘ [- ] process > predict_ncRNA - [- ] process > merge_ncrnas - [e2/7fdb92] process > exonerate_empty_hints [ 0%] 0 of 1 [- ] process > ratt_make_ref_embl - [- ] process > run_ratt - [- ] process > ratt_to_gff3 - [- ] process > transcript_empty_hints - [- ] process > merge_hints - [- ] process > run_augustus_pseudo - [- ] process > run_augustus_contigs - [- ] process > run_snap - [- ] process > merge_genemodels - [- ] process > integrate_genemodels - [- ] process > fix_polycistrons - [- ] process > remove_exons - [- ] process > pseudogene_indexing - [- ] process > pseudogene_last - [- ] process > pseudogene_calling - [- ] process > merge_structural - [- ] process > add_gap_features - executor > local (3) [d4/a2f1c2] process > truncate_input_headers [100%] 1 of 1 ✔ [- ] process > sanitize_input - [- ] process > contiguate_pseudochromosomes - [- ] process > predict_tRNA - [4e/8398aa] process > press_ncRNA_cms [100%] 1 of 1, failed: 1 ✘ [- ] process > predict_ncRNA - [- ] process > merge_ncrnas - [e2/7fdb92] process > exonerate_empty_hints [100%] 1 of 1, failed: 1 [- ] process > ratt_make_ref_embl - [- ] process > run_ratt - [- ] process > ratt_to_gff3 - [- ] process > transcript_empty_hints - [- ] process > merge_hints - [- ] process > run_augustus_pseudo - [- ] process > run_augustus_contigs - [- ] process > run_snap - [- ] process > merge_genemodels - [- ] process > integrate_genemodels - [- ] process > fix_polycistrons - [- ] process > remove_exons - [- ] process > pseudogene_indexing - [- ] process > pseudogene_last - [- ] process > pseudogene_calling - [- ] process > merge_structural - [- ] process > add_gap_features - [- ] process > split_splice_models_at_gaps - [- ] process > add_polypeptides - [- ] process > get_proteins_for_orthomcl - [- ] process > make_ref_input_for_orthomcl - [- ] process > make_target_input_for_orthomcl - [- ] process > blast_for_orthomcl_formatdb - [- ] process > blast_for_orthomcl - [- ] process > run_orthomcl - [- ] process > annotate_orthologs - [- ] process > run_pfam - [- ] process > pfam_to_gff3 - [- ] process > annotate_pfam - [- ] process > make_distribution_gff - [- ] process > make_distribution_gaf - [- ] process > make_distribution_seqs - [- ] process > make_genome_stats - [- ] process > reference_compare - [- ] process > make_tree - [- ] process > blast_for_circos - [- ] process > make_circos_inputs - [- ] process > circos_run_chrs - [- ] process > circos_run_bin - [- ] process > merge_gff3_for_gff3toembl - [- ] process > make_embl - [- ] process > make_report - [- ] process > make_genelist - [- ] process > add_products_to_protein_fasta - WARN: The operator
first
is useless when applied to a value channel which returns a single value by definition -- check channelncrna_cmindex
WARN: Access to undefined parameterTRANSCRIPT_FILE
-- Initialise it to a default value eg.params.TRANSCRIPT_FILE = some_value
WARN: The operatorfirst
is useless when applied to a value channel which returns a single value by definition -- check channelpseudochr_last_index
WARN: Killing pending tasks (1) Error executing process > 'press_ncRNA_cms'Caused by: Process
press_ncRNA_cms
terminated with an error exit status (1)Command executed:
cp /opt/data/cm/rnas.cm ./models.cm cmpress -F models.cm
Command exit status: 1
Command output: (empty)
Command error: cp: cannot stat '/opt/data/cm/rnas.cm': No such file or directory
Work dir: /opt/work/4e/8398aa9b63844d3e9e2108bcaf01e0
Tip: when you have fixed the problem you can continue the execution adding the option
-resume
to the run command line