sanger-pathogens / seroba

k-mer based Pipeline to identify the Serotype from Illumina NGS reads
https://sanger-pathogens.github.io/seroba/
Other
19 stars 16 forks source link

Can't build the ariba database #60

Closed wmiellet closed 2 years ago

wmiellet commented 3 years ago

Hi, After installation with conda install -c bioconda seroba the ariba database doesn't load with seroba createDBs my_database/ 71 because installed tbb=2021.2.0 is incompatible with bowtie2 (for ariba). After downgrading to tbb=2020.2 there is no bowtie2 issue anymore, but I'm getting another error while trying to load the database (see below and attached), namely the prepare_ref issue related to issue #37 despite PR #38, this also result in segmentation fault (core dumped) issue. Any help would be greatly appreciated. See also attached log. False no such file ariba prepareref -f /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/temp_aribaXjuhh_i6n/temp_fasta_ref.fasta -m /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/temp_aribaXjuhh_i6n/temp_meta_ref.tsv --max_noncoding_length 50000 --cdhit_clusters /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/temp_aribaXjuhh_i6n/cdhit_cluster_ref /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/ariba_db/01/ref False

-ci1 -m1 -t1 -fm /home/wmiellet/anaconda3/envs/env-seroba/bin/kmc -k71 -ci1 -m1 -t1 -fm /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/kmer_db/01/01.fasta /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/kmer_db/01/01 /mnt/c/Users/wille/Documents/Pneumococcus/ngsserotyping/db/kmer_db/01 Segmentation fault (core dumped) seroba_log.txt

wmiellet commented 2 years ago

This issue is now resolved.