User story
As a user of the scRNA Core Cell Extraction pipeline, if my cell counting results are bad, I would like to do further counting on more wells from the plate, to try to determine the reason for the bad results (bad samples, isolation failed, or cell counting failed). I would like the LIMS to select the wells and generate the driver file, to avoid user error.
Who are the primary contacts for this story
Abby, Lesley, Katy
Who is the nominated tester for UAT
Abby
Acceptance criteria
To be considered successful the solution must allow:
[x] ~4 wells are selected~ Second wells of all vac tubes are selected.
[x] ~The wells must not have already had cell counting performed on them (can check qc_results table)~ Second wells of all vac tubes are selected; no need to check qc_results table.
[x] ~If possible, the wells should be from different original vac tubes (sample_metadatasupplier_name) to those previously counted~ Second wells of all vac tubes (sample_metadatasupplier_name) are selected.
[x] Hamilton driver csv file produced with columns plate barcode, well position, sample supplier_name, plate_barcode:well_position and sample name, as in other files in linked story. [Well name, i.e. plate_barcode:well_position comes after the sample name]
UAT Updates
[x] Download button: Download Cellaca SECOND Count CSV
[x] Filename: plate ID at the start, e.g. SQPD-12345_hamilton_lrc_pbmc_bank_to_cellaca_second_count.csv
References
This story has a non-blocking relationship with:
Additional context
This is an edge case and was therefore split out of the main story https://github.com/sanger/limber/issues/1653
There is a manual workaround - the file can be manually created / adapted from the 'all wells' file - but there is a risk of user error. The highest risk is that a user selects a well that has been previously selected for cell counting, meaning there is no longer sufficient material in the well for it to continue down the pipeline.
User story As a user of the scRNA Core Cell Extraction pipeline, if my cell counting results are bad, I would like to do further counting on more wells from the plate, to try to determine the reason for the bad results (bad samples, isolation failed, or cell counting failed). I would like the LIMS to select the wells and generate the driver file, to avoid user error.
Who are the primary contacts for this story Abby, Lesley, Katy
Who is the nominated tester for UAT Abby
Acceptance criteria To be considered successful the solution must allow:
sample_metadatasupplier_name) to those previously counted~ Second wells of all vac tubes (sample_metadatasupplier_name) are selected.UAT Updates
References This story has a non-blocking relationship with:
Additional context This is an edge case and was therefore split out of the main story https://github.com/sanger/limber/issues/1653 There is a manual workaround - the file can be manually created / adapted from the 'all wells' file - but there is a risk of user error. The highest risk is that a user selects a well that has been previously selected for cell counting, meaning there is no longer sufficient material in the well for it to continue down the pipeline.