DPL-541 Bioscan OKR: MVP Tag plate creation

[andrewsparkes]

User story As R&D we need an MVP way to create the 24 x 384-well tag plate variants required for a month's set of work on the Bioscan project.

Who are the primary contacts for this story Scott T Emma B Andrew S

Who is the nominated tester for UAT Scott T

Acceptance criteria To be considered successful the solution must allow:

• [ ] New tag layout 'direction' option to get tag 2 indexes in the correct order to match Scotts requirements. Create a variation of the tag layout "InColumnsThenRows" with the tag 2 index calculation changed to (column % scale) * scale + (row % scale)
• [ ] Script to generate the forward (1 x 96 tags) and reverse (24 x 4 tags) tag groups and the 24 tag layout templates using the tag sequences as supplied by Scott. Include a version number in the names so we can regenerate if required. Consider how the tag sequences could be imported into the script. Maybe as files? Consider that a new master batch of tags may include a different layout or different sequences in the tags.
• [ ] Script to create a set of 24 x 384-well tag plates (with a list of the 24 barcodes as output) from the 24 tag layout templates. The output list of barcodes can then be used to manually print off the tag plate barcodes as required. If possible use Baracoda to prefix the barcodes with the tag layout template variation index (1-24) to aid the user (e.g. BSTG-01-nnnnnnnn to BSTG-24-nnnnnnnn). Also consider having this creation script annotate the plate with the tag layout template variation index (1-24) and tag supplier batch reference to aid the user (perhaps as comments visible in Limber similar to the metadata being stored for the LEAN work).

Dependencies None

References This story has a non-blocking relationship with: Other Bioscan OKR stories.

Additional context See the tag plate creation tab in this document. See the research story comments here https://github.com/sanger/General-Backlog-Items/issues/219 for info on the new 'direction' algorithm required.

[andrewsparkes]

Tag plates do not contain aliquots in their wells. The plate itself has a link to the tag layout template. To determine which template was used when a tag plate was created: See qcables table, which links asset (plate) to the lot id. When you create a tag plate, you create a qcables row. Then see the lots table which links the lot to a tag layout template id. Then see tag_layout_templates which links the template to the tag groups for tag 1 and 2, and to the direction and walking algorithms to use. So when the tag plate is used later, the correct tags are applied to the aliquots in the wells which are created to hold the samples transferred into the tag plate from the parent (tag plate is now the child plate).