KatyTaylor
commented
2 months ago Andrew and I just discussed this. We're putting it back into the backlog because it's not ready to be picked up. Reasons are:
- We may be able to re-use the manifest we have already developed for the previous entry point (https://github.com/sanger/sequencescape/issues/3857 - manifest type is called "scRNA Core Pools Plate")
- Question (for Danni?) - do we need to import "retention instructions" for this plate (i.e. is it viewed as a 'stock plate'?)? The answer was "no" for the previous entry point (PBMC pools plate).
- We would like to import sample-level information (pool make-up) for the same reason as was decided for the previous entry point (PBMC pools plate). A few things to check here:
- We discussed the equivalent decision for the previous entry point with HumGen Informatics - who are the people doing the data analysis for this entrypoing? CellGen Informatics?
- Will the existing 10x pipelines work out of the box with sample-level information? (Existing 10x manifests import only pool-level information) If not, this will be a problem for plates coming through SeqOps from the previous entry points too.
- Discuss the contents of this manifest with the relevant SSR & faculty customers (CellGen?), to validate our assumptions and make sure they're happy to fill it out as designed.
N.B. These plates will enter the existing 10X pipeline(s). There is an existing way to get these samples into the LIMS - but, I think we need this new manifest because we need the individual donor information to prevent multiple samples from the same donor being pooled together for sequencing.
User story As a member of the SSR team, who has received a plate from faculty containing cDNA, I would like to be able to import them into Sequencescape along with all the required data.
Who are the primary contacts for this story Lesley, Katy
Who is the nominated tester for UAT Lesley, Liz H?
Acceptance criteria To be considered successful the solution must allow:
References This story has a non-blocking relationship with:
Additional information This will use the normal manifest process - SSRs generate the manifest & barcode labels, send them to faculty, who use the barcodes on their plates and send the manifest back filled in.