satijalab / azimuth

A Shiny web app for mapping datasets using Seurat v4
https://satijalab.org/azimuth
GNU General Public License v3.0
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Is there a way to filter down existing Azimuth reference to tissue/cell of interest? #170

Open TabeaK2 opened 1 year ago

TabeaK2 commented 1 year ago

I am working with the human fetal reference, but my dataset is only comprised of a subset of tissues.

So I wanted to filter the reference dataset to only the cell types of interest before annotating. I can subset the reference object after installing and loading the data, but it doesn't seem to affect the RunAzimuth annotation results - I still get all original cell types. If storing the subset under a new name to use as the reference RunAzimuth fails - due to the specific format for the reference I suspect.

I also tried to create a new reference Azimuth reference from the subset, but haven't gotten that to work either...

Is what I am trying to do feasible? If yes, how do I do it?

cwwon6 commented 9 months ago

Hi,

I also have the same interest in filtering the human fetal reference. I have a few organs I am working with and also would like to only observe the immune cell types only, as the other cell types are interfering with the identification on my immune subset of analysis.

Is there any way of filtering for particular organs and cell types ?

Cheers, Chee

Chiranjit1504 commented 1 month ago

Hi,

Did you get this to work?